Method development and validation for the estimation of VORICANOZOLE by RP-HPLC

Abstract

A reverse phase high performance liquid chromatographic method is been used for the Validation of voriconazole. The chromatographical condition trial carried out on a Column Symmetry C18 (4.6 x 150mm) with the particle size of (5μm). The mobile phase use is of methanol,0.1%OPA with (pH 3.0) i.e. acidic and water (40+60% v/v). (0.7ml/min) is the flow rate and the following detection was carried out at 295nm. The retention time was 10min. The parameters like Linearity, robustness, accuracy, precision is within the specific limit as per ICH guideline. The Symmetry, plate number and RSD are as per limit and satisfactorily low which is required. This analytical experiment proved to be accurate, reliable, reproducible and consistency in its results. So, this proposed method can be successfully utilized in estimation of voriconazole in any quality control of Bulk dosage form and pharmaceutical formulations.